Isopod Primers

The primer sequences used in my isopod dissertation research are below. Scott France kindly made the 16S primers available; the 12S primers are based on Daphnia (Lehman et al. 1995/Hanner & Fugate 1997), and the COI primers are the Folmer et al. 1994 primers.

Regina Wetzer

16SAR-SF, PR100, 5' - CGC CTG TTT ATC AAA AAC AT - 3'
16SBR-SF, PR101, 5' - CCG GTT TGA ACT CAG ATC ATG - 3'

12SCRF, PR102, 5' - GAG AGT GAC GGG CGA TAT GT - 3'
12SCRR, PR103, 5' - AAA CCA GGA TTA GAT ACC CTA TTA T - 3'

COIF, PR115, 5' - TCW ACN AAY CAY AAR GAY ATT GG - 3'
COIR, PR114, 5' - ACY TCN GGR TGN CCR AAR ARY CA - 3'

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Lehman, N., Pfrender, M. E., Morin, P. A., Crease, T. J., Lynch, M. 1995. A hierarchical molecular phylogeny within the genus Daphnia. Molecular Phylogenetics and Evolution 4:395-407.

Folmer, O., Black, M., Hoeh, R., Lutz, R., Vrijenhoek, R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3(5):294-299.

Hanner, R., Fugate, M. 1997. Branchiopod phylogenetic reconstruction from 12S rDNA sequence data. Journal of Crustacean Biology 17(1):174-183.

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